Human Immunodeficiency Virus HIV-1 Western Blot
HIV-1 Western Blot
Specimen Collection Instructions
Blood should be collected in a plastic, sterile STD Program approved collection tube. Please follow the manufacturer's instructions on clot time and centrifuge speed/time requirements.
The HIV Laboratory Test Requisition form must be filled out completely. The following information must be included on the form:
Storage and Transport Instructions
Speciments should be shipped refrigerated (2-8ºC) or ambient (8-37°C).. Specimens can be stored for up to 7 days. For longer storage, specimens must be frozen at -20ºC or colder. Frozen specimens must be shipped on dry ice and received at a temperature of -20ºC or colder.
Causes for Rejection
Limitations for the Procedure
Testing alone cannot be used to diagnose AIDS, which is a clinical syndrome. The diagnosis of AIDS must be establised clinically. Indeterminate immunoblots should not be used as the sole basis of HIV-1 infection. Persons with an indeterminate immunoblot should be retested using a fresh specimen after 6 months. A negative immunoblot does not exclude the possibility of exposure to or infection with HIV-1. A person who has antibodies to HIV-1 is presumed to be infected with the virus, except that a person who has participated in an HIV vaccine study may develop antibodies to the vaccine and may or may not be infected with HIV. Clinical correlation is indicated with appropriate counseling, medical evaluation and possibly additional testing to decide whether a diagnosis of HIV infection is accurate.