Louisiana Department of Health & Hospitals | Kathy Kliebert, Secretary

Statewide Initiatives

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Human Immunodeficiency Virus Type 2 EIA

HIV-1/2 + O EIA

  • Laboratory Location – Central Laboratory
  • CPT Code – 86701
  • Synonyms – HIIV 2, Anti-HIV 2
  • Test Description - The HIV-2 EIA is used to detect antibodies to HIV-2 in human serum or plasma. It is used as an aid in the diagnosis of infection with HIV-2. HIV-2 EIA is tested when the HIV 1/2 EIA is repeatedly reactive and the HIV-1 Western Blot is negative.

Possible Results

  • Non-Reactive
  • Reactive

Reference Range

  • Non-Reactive

Specimen Requirements

  • Specimen Type - Serum
  • Specimen Container(s) - red top tubes, Marble top tubes, polypropylene vials
  • Minumum accepted volume - 209 µL(does not allow for repeat testing)

Specimen Collection Instructions

Blood should be collected in a plastic, sterile STD Program approved collection tube. Please follow the manufacturer's instructions on clot time and centrifuge speed/time requirements.

The HIV Laboratory Test Requisition form must be filled out completely. The following information must be included on the form:

  • Patient's Name
  • Patient's Date of Birth
  • Patient's Gender
  • Patient's race/ethnicity
  • Date and time of specimen collection
  • Test requested
  • Type of specimen collected
  • Submitter's name and address
  • External ID#

Storage and Transport Instructions

Speciments should be shipped refrigerated (2-8ºC). Specimens can be stored for up to 7 days. For longer storage, specimens must be frozen at -15ºC or colder. Frozen specimens must be shipped on dry ice and received at a temperature of -15ºC or colder.

Causes for Rejection

  • Grossly lipemic, grossly hemolyzed, and icteric specimens must be rejected
  • Improper labeling
  • expired collection tubes
  • unspun samples
  • tubes that contain less than 90% of the total drawing capacity (QNS)
  • Specimen age greater than 7 days if specimen has not been frozen
  • Improper storage or improper transport temperature requirements are also reasons for rejection

Limitations for the Procedure

Repeatedly reactive specimen must be investigated by additional, more specific or supplemental tests. Testing alone cannot be used to diagnose AIDS, which is a clinical syndrome. The diagnosis of AIDS must be established clinically. A negative test result at any point does not preclude the possibility of exposure to or infection with HIV-2. HIV-1 may cause cross reactivity with the HIV-2 EIA, because both viruses contain similar protiens.

Interfering Substances

  • Grossly hemolyzed, lipemic, or icteric specimens


  • BioRad Genetic Systems™ HIV-1/HIV-2 Plus O EIA package insert
  • BioRad Genetic Systems™ HIV-2 EIA package insert
  • EVOLIS™ Operator Manual


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