Louisiana Department of Health & Hospitals | Kathy Kliebert, Secretary

Statewide Initiatives

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Rubella IgG EIA

Syphilis IgG EIA

  • Laboratory Location – Central Laboratory
  • CPT Code – 86762
  • Synonyms – Rubella IgG, German Measles
  • Test Description - The Rubella IgG EIA is used to aid in the assessment of the patient's immunological response to rubella, and as a qualitative screening test to detemine the immune status of individuals, including women of childbearing age.

Possible Results

  • Negative
  • Equivocal
  • Positive

Reference Range

  • Positive

Specimen Requirements

  • Specimen Type - Serum
  • Specimen Container(s) - red top tubes, Marble top tubes, polypropylene vials
  • Minumum accepted volume - 216 μL

Specimen Collection Instructions

Blood should be collected in a plastic, sterile STD Program approved collection tube. Please follow the manufacturer's instructions on clot time and centrifuge speed/time requirements.

The Virology Lab 96 form must be filled out completely. The following information must be included on the form:

  • Patient's Name
  • Patient's Date of Birth
  • Patient's Gender
  • Date and time of specimen collection
  • Test requested
  • Type of specimen collected
  • Submitter's name and address

Storage and Transport Instructions

Specimens should be shipped refrigerated (2-8ºC). If specimens are not tested within 8 hours, they should be stored at 2-8°C for up to 48 hours. For longer storage, specimens must be frozen at -20ºC or colder. Frozen specimens must be shipped on dry ice and received at a temperature of -20ºC or colder.

Causes for Rejection

  • Improper labeling
  • expired collection tubes
  • incorrect specimen type
  • heat inactivated, hemolyzed, gross contamination, and icteric specimens must be rejected.
  • unspun samples
  • tubes that contain less than 90% of the total drawing capacity (QNS)
  • Improper storage or improper transport temperature requirements are also reasons for rejection

Limitations for the Procedure

The results obtained with the Rubella IgG EIA test serve only as an aid to diagnosis and should not be interpreted as diagnostic themselves. A single positive result only indicates previous immunologic exposure; the level of antibody response or class of antibody response may not be used to determine active infection or disease stage. Paired specimens should be collected during the acute and convalescent stages of infection, and tested concurrently to detect significant antibody increases. The acute phase sample should be collected early in the infection, preferably within 7 days of the onset of symptoms, and the convalescent phase sample 1-2 weeks after the first sample, but not earlier than 10 days after the onset of symptoms.

Interfering Substances

  • Grossly hemolyzed, icteric specimens, or gross contamination


  • BioRad Rubella IgG EIA package Insert
  • EVOLISTM Operator Manual


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